A SPECTRUM OF FREE SOFTWARE TOOLS FOR PROCESSING THE VCF VARIANT CALL FORMAT: VCFLIB, BIO-VCF, CYVCF2, HTS-NIM AND SLIVAR.

A spectrum of free software tools for processing the VCF variant call format: vcflib, bio-vcf, cyvcf2, hts-nim and slivar.

Since its introduction in 2011 the variant call format (VCF) has been widely adopted for processing DNA and RNA variants in practically all population studies-as well as in somatic and germline mutation studies.The VCF format can represent single nucleotide variants, multi-nucleotide variants, insertions and deletions, and simple structural variant

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Wide Field Spectral Imaging with Shifted Excitation Raman Difference Spectroscopy Using the Nod and Shuffle Technique

Wide field Raman imaging using the integral field Nightmare Before Christmas spectroscopy approach was used as a fast, one shot imaging method for the simultaneous collection of all spectra composing a Raman image.For the suppression of autofluorescence and background signals such as room light, shifted excitation Raman difference spectroscopy (SER

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